s.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can
nt: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl
alcohol) and after sectioning you can use dishwasher s
...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] på vegne af Lori Gemeinhardt
[loreli...@mac.com]
Sendt: 18. april 2014 02:18
Til: histonet@lists.utsouthwestern.edu
Emne: [Histonet] Xylene substitute for processing GI biopsies
Hi there!
I have spent a lot of time reading the archives, but still
Hi Lori,
Pretty much any xylene sub will work for processing tissues and staining. You
may need to add additional time in the "xylene" steps, since the action of the
subs isn't as quick as xylene.
In addition, the substitutes are more intolerant of water. How humid is your
lab? If it's dry, the
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Friday, April 18, 2014 10:41 AM
To: 'Blazek, Linda'; Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene substitute for processing GI biopsie
tonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene substitute for processing GI biopsies
Lori,
We use Formula 83 from CBG for both processing and staining. We have used this
for years with no problem for our GI biopsies. It wouldn't do any good to
share my processing schedule with
n.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies
Hi Lori:
Instead of xylene you can use isopropyl (also known
Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl
alcohol) and after sectioning you can use dishwasher soap before going directly
to the staining procedure. After staining you need nothing but an oven to dry
out the stained sections and go diractly to coversli
Hi there!
I have spent a lot of time reading the archives, but still need some specific
and current input/advice.
I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with
a xylene substitute. I need insight on protocols, brands, effects on
staining. The good, the bad
g tween in the secondary hyb.solution
> To: "histonet@lists.utsouthwestern.edu" >
> Date: Monday, March 5, 2012, 10:25 AM
>
>
> Hi,
>
> I currently did an experiment in which I left out Tween in de
> hybridisation solution of
> the secondary antibodies. I only made a solu
We use Formula 83 for overnight processing. It works well with no
problems for special staining or IHC. The only problem is that the
tissues do have to be well fixed before processing.
I have also used Americlear (to which I now have an allergy) and Sub-X
and had good results with both at o
Kelly,
We uses for years mineral oil instead xylene for
overnight manual processing and have seen not any
adverse effect in H&E, HC and IHC.
Opposite, we noticed, that our slides becomed better,
crisp and clear than with xylene.
Mineral oil have also many positive additional moments for
techs and l
Greetings Histonet!
Does anyone use xylene substitutes for routine over-night processing? If so,
what do you use and does it have any adverse effect your special stains?
Thank you in advance for your help,
Kelly
Kelly S. Cross B.S., HT (ASCP)
Medical Laboratory Supervisor
Veterinary Pathobiolog
13 matches
Mail list logo
