...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Wednesday, February 03, 2010 3:49 PM
To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] TRAP assay - acid phosphatase
We have used it on FFPE EDTA decaled mouse bone, with a
@lists.utsouthwestern.edu
Subject: Re: [Histonet] TRAP assay - acid phosphatase
Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on
mouse tibia for IHC.
If so, 1) can you do it on paraffin and/or frozen sections?
2) if you do it on frozens, do you decalcify first?
3
Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on
mouse tibia for IHC.
If so, 1) can you do it on paraffin and/or frozen sections?
2) if you do it on frozens, do you decalcify first?
3) what type of blade do you use for sectioning on the cryostat?
