Ventana has a kit for alcian blue. I wasn't very satisfied with this kit. The staining was faint in comparison to manual staining. Staining temp is at 60 degrees. I'm not sure how the temp influences exactly the pH of the staining solution, but it may decrease it under the wanted 2,5. So the result is rather a pH1 than pH2,5 alcian blue. That are just theoretic considerations, never checked. - what do you think? Gudrun
-----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Scott, Allison D Gesendet: Freitag, 21. Oktober 2011 16:01 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus Hello to all in histo land. For those of you that have the ventana special stains machine, can the alcian blue 2.5 be done on the machine? I am not sure if they have kits for this stain. Also is anyone using the cryoembedding apparatus from pathology innovations? Thanks in advance. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet