Phyllis, we mainly don't have this problem and have been using the ultraview for at least one year. Do you airdry the slides horizontally? We only have the problem of loosing tissue, when the water under the sections was not moved away well enough. And we often start the run directly after cutting - no longer airdrying or oven. I would recommend to put the slides directly after cutting in the 60°C oven and dry vertically.
Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Phyllis Thaxton Gesendet: Mittwoch, 08. April 2009 15:44 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Benchmark XT Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
