We work with the Benchmark XT, ultraview kit (polymer), CC1 retrieval (pH
8-9) 30 min, C4d from Zytomed 1:25 32min, with amplification.
Gudrun Lang
Akh Linz, Austria
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
Hi César
we use mo-a-C4d, clone CL314 (Quidel A213) at 1.25 ug/ml (1:800) for
cryosections of kidney biopsies (indirect immunofluorescence), it won't work
on FFPE material (at least in our hands). For FFPE, Biomedica's rb-a-C4d
(BI-RC4D) works fine at ca. 6 ug/ml (1:30) after HIER in a high pH
Our's is from Zytomed, a polyclonal rabbit (a12-5000). We work with the
benchmark XT, 1:25 with amplifier.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Cesar
Francisco Romero
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