We avoid to put specimens of different size into one cassette in general (especially with biopsies). If I have too pieces of different size with a clear cut side, I put them both on the ground of the mold. I think one could never precisly put a specimen in a certain level into the mold, if it doesn't lie on the bottom. Gudrun
-----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sanjeet Gesendet: Donnerstag, 31. Juli 2014 03:44 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fwd: SOP on embedding tissues of different sizes in same mold Sent from my iPhone Begin forwarded message: > From: Sanjeet <asanj...@yahoo.com> > Date: July 30, 2014 at 9:15:25 PM EDT > To: "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> > Subject: SOP on embedding tissues of different sizes in same mold > > Hi > Does anyone in the Histo world have an SOP on how to embed tissues of different sizes in the same mold. Do you embed in different levels depending upon the size, the larger embedded first and the smallest at the end. Or do you embed all in the same plane regardless of the tissue size. Are you able to justify and salvage all tissue on the slide. > Thank you all in advance > Sanjeet > > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet