Re: AW: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue

2013-05-28 Thread Krista Sider
Hi Gudrun How deep would you consider the oxidation to go? I have tried samples from around 200um into the block. The Alkaline Alcohol converts the alcian blue to monastral fast blue, which is insoluble - this is what i have been told. So locks it in I guess. Doesn't really matter as I don't get a

AW: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue

2013-05-28 Thread Gudrun Lang
Hi Krista, Maybe the bad stainability is more due to oxidation by air of the old block-surface than due to fixation. A deeper slide for comparison would show. I don't know what the alkaline alcohol is good for ? Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.e