We do an automated doublestain, that works with Peroxidase-DAB and Alk. Phosphatase-Red. The critical point is, to perform a second HIER-step between the two detection-sequences. The fixed specimen don't suffer from the second heating, but the native antibodies are destroyed and are not detectable with the second-secondary-antibody. DAB sticks so well to the tissue, that it isn't washed away by this treatment.
The nice think of this method is, that you don't have to think about species. Gudrun Lang -----Ursprüngliche Nachricht----- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Edwards, R.E. Gesendet: Montag, 03. November 2008 15:09 An: histonet Betreff: [Histonet] double trouble OK chaps, which in your opinion is the best double labelling method when one is using primary antibodies raised in the same species, I am aware that Vector labs supply such a kit; many thanks. Cheers Richard Edwards University of Leicester U.K.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet