Sally, I added SubX to my acrymount since I have plenty of that in stock. It worked!! And I am so glad I can use up what I have instead of having to purchase a case of Sub X glue. My slides look clear and film free. I now am more confident in passing these slides over to be read.
Thanks for all the helpful suggestions everyone! -----Original Message----- From: Breeden, Sara [mailto:sbree...@nmda.nmsu.edu] Sent: Tuesday, March 30, 2010 11:25 AM To: Vanessa Avalos Subject: RE: [Histonet] Coverslipping with SubX Try thinning out the Sub-X-based mounting medium with some Sub-X. It sounds as if your mounting medium is too thick. Try that and see if it helps. You want your mounting medium to drip like cheap pancake syrup would drip - not too thick and not too thin. Too thick makes bubbles. I use Sub-X on my processor but use xylene on my autostainer, but that's because I have an tape coverslipper. I know you mean you're coverslipping by hand but see if the thinner medium helps you. Let me know! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet