> Last comment on the paraffin conversion for me. > > You can use bees wax if you want in your processor and embedder that doesn't > mean you going to get the optimal results for every situation. Paraffin > types are a personal preference for most people or labs. It's a little more > hassle to keep two paraffins on hand instead of just one.. Low polymer > paraffins penetrate better, faster, so and so forth and the higher polymer > paraffins take a little longer but supports tissue better for sectioning. > There are positives and negatives for both. Using one paraffin type is > fine, all personal preference. We could go on forever with this!! > > > I am not endorsing any paraffin on here, used many different paraffins and > each work as anticipated. > > Thanks, > > Tim > > > > > Sent: Monday, July 11, 2011 5:44 AM > Subject: Re: [Histonet] Re: Embedding Media > > > Amita, > > The paraffin that we use is also a Leica product. It was formerly sold under > the Surgipath label, but since to companies have merged together, it now > sells under the Leica name. It is a "beaded" pellet paraffin and has a > version suitable for infiltration and a version suitable for embedding. The > box of the embedding paraffin suggests it can be utilized for *both* > purposes, however we do not choose to use it that way. So yes, we use two > different types of paraffin. > > Hope that helps! > Michelle > > > > I continuation of same discussion , i would like to have clarification for > > our lab..we use ....beaded paraffin "Leica Paraplast" both for processing > > and embedding . Do you advice us to use different paraffin for both steps? > > > > > > Paula, Please share if you get any feed back on Richard Allan product. > > > > Amita > > > > > > > Subject: [Histonet] Re: Embedding Media > > Sent by: histonet-boun...@lists.utsouthwestern.edu > > > > > > > > Hi Paula, > > > > Obviously there are no "compression free" paraffin's on the market. Look > > for a paraffin with a higher polymer content, this makes the paraffin > > firmer > > and less likely to compress. Other factor for into section compression > > but > > paraffin is a good place to start. > > > > The higher polymer paraffin's are not as suited for processing, it is more > > for the embedding portion of the process. > > > > Use a paraffin with lower polymers content in the processor and higher for > > embedding and you will like the results. > > > > > > Tim > > > > > > > Subject: [Histonet] Embedding Media > > To: <histonet@lists.utsouthwestern.edu> > > > > > Hello, > > > > > > > > We are considering a switch to a different brand of paraffin and this is > > because I feel we are having too many compressions in some of our tissue > > sections. Currently, we use Tissue Path Paraplast, regular. > > > > > > > > I would like to get feedback from you as to what you prefer. Looking on > > line, Richard Allan has a product called Signature Series Paraffin that > > comes in a type L that offers compression-free sections and I was also > > hoping to get any feedback on that product. > > > > > > > > I would greatly appreciate any suggestions and thoughts. > > > > Thanks in advance, > > > > Paula > > > > > > >
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