We once had this problem..We routinely filter our hematoxylin every morning at startup.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burrage, Shannon L. Sent: Tuesday, February 11, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] RE: [Histonet] Purple haze on H&E slides Are you using acid alcohol, and Harris Hematoxylin? Are you filtering the heamatatoxylin every day? We still use a very small amount of gelatin in the waerbath. And get no background stain. So many veriables here. Good luck, Shannon Burrage shannon.burr...@unitypoint.org ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Campbell, Tasha M. [tmcampb...@fmh.org] Sent: Monday, February 10, 2014 7:37 PM To: Huggins, Haley - MRMC Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple haze on H&E slides What brand of reagents are u using and what is your protocol? What slides r u using and r u using gelatin in ur waterbath? Tasha Sent from my iPhone > On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" <haley.hugg...@dignityhealth.org> wrote: > > I am trying to get the histology lab up and running and I am having difficulties getting the H&E Stain just right. I am nearly there, but I keep getting a purple bleed or haze around the tissue. Any suggestions on getting rid of this? I actually got rid of it at one point, but the medical director wasn't happy with the stain. I appreciate any suggestions from the histology world. > > Haley Huggins > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet