RE: [Histonet] ?bacterial/fungal contamination on slides

2008-09-19 Thread Monfils, Paul
Fungi can grow in a number of different aqueous stain solutions. One time a tech brought me a slide she had stained with GMS for fungi, together with the positive control slide she had run. I could see fungi on the test slide but there was no sign of silver staining. The control slide showed po

RE: [Histonet] ?bacterial/fungal contamination on slides

2008-09-19 Thread Weber, Susan (VHACLE)
Have you checked your water source for contamination? We culture our DI water regularly. Make sure you are culturing from the tap you actually obtain the water from. We had this problem at another hospital a few years ago and found contamination at the tap. Hope this helps. Susan M Weber HT(ASCP)

RE: [Histonet] ?bacterial/fungal contamination on slides

2008-09-18 Thread Laurie Colbert
We see this every once in a while, and have traced it to the water stations on our stainer. They are supposed to be cleaned with bleach once a week, but that wasn't happening. Another time, we had contamination on the spigot of our 5 gallon deionized water bottle. We are supposed to clean that w

RE: [Histonet] ?bacterial/fungal contamination on slides

2008-09-18 Thread Tom McNemar
We had a similar problem awhile back and traced it to our precut control slides. We cut certain control slides ahead and store them in slide boxes. We started seeing fungus where it should not have been. We were just cutting the control slides, putting the wet slides into the boxes, covering