Re: [Histonet] Acceptable way to "bake" sections onto slides?

2010-08-13 Thread Gibson, Philip
Hi Everyone Just for clarification; our current practice involves a mixture of placing racks of slides in an oven for 25 minutes, or using the hot area of an autostainer (we're talking H&E's here, BTW). Occasionally, with a particularly urgent case us old-timers will place individual slides di

RE: [Histonet] Acceptable way to "bake" sections onto slides?

2010-08-13 Thread Podawiltz, Thomas
...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 13, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu; PhilipGibson Subject: Re: [Histonet] Acceptable way to "bake" sections onto slides? The correct answer is "b": if there is water underneath the section that is

Re: [Histonet] Acceptable way to "bake" sections onto slides?

2010-08-13 Thread Rene J Buesa
The correct answer is "b": if there is water underneath the section that is going to be placed on the hot plate, the most likely artifact is that of "empty nuclei" that will ruin the sections' usefulness. René J. --- On Fri, 8/13/10, Gibson, Philip wrote: From: Gibson, Philip Subject: [Histo

Re: [Histonet] Acceptable way to "bake" sections onto slides?

2010-08-13 Thread annigyg
Use the sakura DRS hotpot Loading from the plate (apart for the other drawbacks) will set the wax on the first slides put in the racks and you may end uo with inadequate dewaxing Just my 5cents worth Annieinarabia Empower your Business with BlackBerry® and Mobile Solutions from Etisalat -Or