This is a well known phenomenon of amyloid staining and it is fairly
common. It is due to the fact that amyloid is a very variable material
and different samples may react differently to the dyes. I suggest you
restain using Highman's congo red method
What protocol and reagents are you using for the stain?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com
On 1/23/15, 10:43 AM, Jeffrey Robinson
jrobin...@pathology-associates.com wrote:
Greetings to all
We do all of our Congo Red staining on Muscle and Nerve frozen sections.
Sometimes when there is very little amyloid detected using the
bright-field/polarizing microscope our pathologist will confirm under a
fluorescent microscope with the Rhodamine Red filter. This shows the amyloid
