Re: [Histonet] Best Carmine stain

2013-03-20 Thread Lee & Peggy Wenk
If you are looking for glycogen - so a PAS. More sensitive and a deeper magenta color than the Best Carmine. Curious, what is "without water"? - The Frozen section? But there's water in the cells, which is what is freezing. - The stain?? But most stains are made in water (aqueous stain

RE: [Histonet] Best Carmine stain

2013-03-17 Thread Smith, Allen
Best's carmine and mucicarmine are very different stains. Mucicarmine is carminic acid mordanted with aluminum in acid solution. It stains mucus but not glycogen. Best's carmine is an ammoniated carminic acid, probably an amine at one end and an amide at the other. It is very basic. It is a se