Try cooling down your water bath with ice before you cut the brai= n.
All CNS tissue will act like you described. You may end up w= ith
more wrinkles with the cooler bath, but put the slides in a 70C oven
fo= r about an hour and they will usually fall out. =)
Happy Cuttin=
The fixation time is too short.
Fix as long as you can (how about 2-3 days at least for brain?)
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and
