Shattering is not produced at the embedding section. It is caused during
processing specially because of improper dehydration. If the LN are too thick
to begin with, it will also contribute to improper dehydration and as a
consequence poor infiltration.
René J.
--- On Mon, 4/27/09, Greg Dobbin
Greg,
Are you sure no one is using freezing spray when sectioning? The effect of
freezing spray on the block would yield the appearance you describe.
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Greg Dobbin
Sent: Mon 4/27/2009 2:04 PM
To:
I am positive no freezing spray being used.
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
I find that the harder I work, the
more luck I
: [Histonet] Cracked or shattered LN sections
I am positive no freezing spray being used.
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
I find
