RE: [Histonet] Cutting paraffin sections in a warm room

2009-11-05 Thread gayle callis
Adam, Cool trimmed bone blocks on a block of ice with extra water on top of ice. This helps soften the bone and keepS the block cold but don't over soak, that can lead to shredded sections. A cube of ice wrapped in gauze, held to face of block and also held to knife holder may help or simply

Re: [Histonet] Cutting paraffin sections in a warm room

2009-11-05 Thread louise renton
One of the little things I learned along the way regarding bone sectioning: if time allows, trim (face) the blocks and leave them in the deep freeze (-?20) overnight. T Then when you are ready to section, take ONE block out at a time and place on ice. this trick is especially useful for us as we