We don't destain them! We remove the coverslip and bring them down to water again with the controls you will be running. We then run them on the Bond with out the Bake and De-wax step. The stain gets removed mostly when you bring them to water again. Our Pathologists have not complained when we do this!
Good Luck, Josie Britton HT Cheshire Medical Center Keene, NH -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Virginia Chladek Sent: Monday, March 05, 2012 3:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Destain/Restain **************************************** I need your help- My doc needs me to destain an h&e slide to run a ck5 on the Bond3, how can I do this without the tissue falling off? I ran the slide back down from xylene to water and destained, then introduced the slide to bond wash before starting the protocol in bond wash again and lost the tissue. Any suggestions? Thanks! Maggie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
