> Date: Fri, 16 Jul 2010 11:00:01 -0500
> From: mpe...@grhs.net
> To: kgrob...@rci.rutgers.edu; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Embedding multiple GI pieces on end in a paraffin
> block
> CC:
>
> I have techs that embed 10-15 pieces on end in
To all,
OK, looks like most of you are saying the same thing-work fast! :o) I've
printed out all of your replies and discussed them with the graduate
student who needs this done for her thesis, and she agrees with me-it's
just going to take practice. I'll try the "cool slowly/work fast"
suggest
We regularly embed 6-12 pieces on end in one block without any special
method. You just have to be quick... and don't leave the block on the cold
plate very long... just touch the cold plate briefly while embedding the
individual piece, then lift the block off the plate until you grab the next
pie
I have techs that embed 10-15 pieces on end in one block. Just cool the
block slowly and move your pieces around quickly.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
kgrob...@rci.rutgers.edu
Sent: Friday
How many are we talking about? I embed 6 sections of mouse = bowel on
end and it works? Just fill the mold, put it on the cold spo= t for a
second, then on some room temperature area, the paraffin will harde= n
slowly enough that you should be able to embed them?
Sarah Goebel, B.
