You could try hydrogen peroxide - it wil get rid of the heme in the RBCs which is probably giving you the background. I would try a 1:10 dilution (or higher) of the H2O2 (in PBS) that you usually get from the store/pharmacy - undiluted will probably bubble too much and lift off the tissue. You will have to try dilutions and time - usually 10 minutes is good, but with a diluted solution you may need to go longer. Then wash the slides in PBS to get rid of the H2O2. Good Luck Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of girish cm Sent: Tuesday, December 11, 2012 12:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Enquiry about blood removal from frozen tissue sections Hello, I would like to know whether any methods (other than cold PBS wash) or products are available to remove the blood components from frozen tissue sections. My application is antibody targeted spectroscopic imaging in which the blood components are providing background noise. Thanking you, Regards -- Girish C M Senior Research Fellow Amrita Centre for Nanoscience & Molecular Medicine Amrita Institute of Medical Sciences & Research Centre Cochin, Kerala India-682 041 Ph: 9645095045 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
