I have seen this happen with high levels of Isopropanol in alcohols after the
eosins, I switch to regular reagent alcohols and seemed to eliminate the
bleeding of the eosin.
Michael
Michael R. LaFriniere, HT (ASCP)
Executive Director
Capital Choice Pathology Laboratory
12041 Bournefield
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, 20 June 2014 10:11 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson';
'histo...@listsutsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching
Is your
...@lists.utsouthwestern.edu] On Behalf Of Sanders,
Jeanine (CDC/OID/NCEZID)
Sent: Thursday, June 19, 2014 5:26 PM
To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching
Have you changed your coverslipping mountant?
-Original Message-
From: histonet-boun
@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching
Have you changed your coverslipping mountant?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Thursday, June 19, 2014 5
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Friday, June 20, 2014 8:15 AM
To: Rathborne, Toni
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin Leaching
We haven't changed our mounting medium, but I'll
We had the same problem. Increase your Alcohol/Reagents (following Eosin) times
as well as using fresh ones. I use 80% for about 20 sec and (2)100% for a
minute each and (3) Xylene Subs for 2 min each. I also use a fresh 80% and
rotate the 100% each day. If using a Xylene Sub it could also be a
Have you changed your coverslipping mountant?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject:
Your xylene and/or alcohols may need changing.
Janet
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Scott Hendricksen
Sent: Wed 2/3/2010 9:02 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections
Hi
Humidity has played a large part in our slides leaching Eosin. We have a
dehumidifier in our staining room and we also use H2Blue Beads in our Xylene
substitute. The beads absorb water from the substitute and help prevent
leaching of the stain.
The beads are ordered from Amercian Mastertech.
Where do you get the beads from?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Joyce
Cline
Sent: Wednesday, February 03, 2010 2:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Eosin
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