I have used the ULYSIS Nucleic Acid Labeling Kit from Invitrogen. They make it with many different fluorochromes, I have used the AlexaFluor 488 and AlexaFluor 594 with good results.
Joseph A. Jurcisek Senior Research Associate Center for Microbial Pathogenesis The Research Institute at Nationwide Children's Hospital 700 Children's Dr. Columbus, OH 43205-2696 (614) 355-3521 fax (614) 722-2818 joseph.jurci...@nationwidechildrens.org -----Original Message----- From: FU,DONGTAO [mailto:f...@ufl.edu] Sent: Tuesday, August 25, 2009 2:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FISH probe labeling Hi, All We are going to do FISH on mouse tissue. But we have to fluorescent label cDNA probe(~2kb in length) by ourselves because there is no commercial probe is available. Does anyone give me some suggestions? Like which company's product is suitable for labeling DNA probe? Which labeling methods is better? End labeling or multi? Thank you, Ann Dongtao Fu Dept. of Pathology University of Flodrida Gainesville, FL 32610 ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
