RE: [Histonet] Fatty Fixation

2013-06-24 Thread McAnn, Sherrian
We use Dissect-Aide seems to work really well especially with lymph nodes too. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, June 20, 2013 1:23 PM To:

RE: [Histonet] Fatty Fixation

2013-06-24 Thread Sarah Dysart
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Friday, June 21, 2013 3:19 PM To: White, Lisa M.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fatty Fixation What exactly is wrong with the fatty specimens? If the nuclei look smudgy

Re: [Histonet] Fatty Fixation

2013-06-21 Thread Lee Peggy Wenk
What exactly is wrong with the fatty specimens? If the nuclei look smudgy, with no nuclear detail, then it has not been fixed long enough. If the fat is still in the tissue and you cannot section it on a microtome, then the tissue has not had enough time during processing, especially length

Re: [Histonet] Fatty Fixation

2013-06-20 Thread Rene J Buesa
The answer is time, time, time (36h) René J. From: White, Lisa M. lisa.whi...@va.gov To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 20, 2013 2:23 PM Subject: [Histonet] Fatty Fixation Does anyone have a method they will share to fix fatty specimens?  Does anyone utilize a stir

Re: [Histonet] Fatty Fixation

2013-06-20 Thread Massimo
; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Inviato: Giovedì 20 Giugno 2013 22:38 Oggetto: Re: [Histonet] Fatty Fixation The answer is time, time, time (36h) René J. From: White, Lisa M. lisa.whi...@va.gov To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 20, 2013 2