It must be completed in seconds. Buried in powdered dry ice is one way. Immersion in a slurry of dry ice and isopentane is another. Cordially, Charles W. Scouten, Ph.D Product Specialist, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com <http://www.myneurolab.com/> www.leica-microsystems.com <http://www.leica-microsystems.com/> IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone <a...@buffalo.edu> Sent: Tuesday, May 17, 2011 9:39 AM To: <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Freezing rodent brain How is everyone freezing hippocampus sections? The current method being used seems to be causing a lot of artifact. Thanks
Annette Featherstone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet