Sheila, We use CM's HSV I (361A) and HSV II (362A) and cocktail them for use on VMS's BenchMark and XTs. I use them cocktailed to achieve a 1:100 dilution of both with mild CC1 @ 42 degrees C for 32 minutes with AB block and Hem II/8 minutes.
Hope this helps, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, June 07, 2011 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HSV I and II Good day Histonetters everywhere! I was wondering if anyone could give me some brilliant advice. We are currently running HSVI and HSVII antibodies from Cell Marque that are still ASR status. My pathologist wants me to cocktail the two together. Does anyone have any experience with this and could you give me any advice? All help would be greatly appreciated. Can I make a 50/50 mixture of the RTU antibodies and use my longest protocol? We are using the Ventana Ultra. Thank you so much. Sheila Fonner, HT (ASCP) KDL Pathology Knoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet