Gudrun:
Were the cases to be known as positive, or were you trying to determine just
that?
If they were known as (+) then the bacteria should be seen outside the cells.
If you were trying to determine if they were (+) and you do not see the
organisms it is just that those cases were (-), especial
Hello,
We use what is pretty much a diff quik stain, the stain is called QW and it
is from poly scientific. The procedure is really easy. Deparaffinize,
alcohols, methanol, water and then we do 15 slow dips in the QW2, 15 slow
dips in the QW3, wash in water, 2 quick dips in alcohol and then dry
We basically do a dif quik stain. I buy a kit called "Three Step Stain"
from Cardinal. The total staining process takes about 1 minute.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M
We use an Acridine Orange stain. You will need a fluorescent scope for it,
though. It's a very simple procedure. Depariffinize slides, air dry, apply AO
solution, let stand for 7 minutes, rinse, dry, coverslip from xylene.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.
We use Cell Marque's Polyclonal H. Pylori (CMC435) at a 1:100 dilution on the
Ventana BenchMark for 32 minutes, Protease 1 for 12 minutes.
~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson, AZ
~Don't let your life end before it begins~
OU Rocks
__
We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20
minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare)
10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse
probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes
hematoxylin(Dako).
Hi Chakib,
We use Ventana's (manufactured by Cell Marque) H. Pylori on their instruments.
The protocol is specific to the instruments but involves a routine HIER step
and Avidin/Biotin blocking. The antibody itself is ready-to-use but is
available from Cell Marque as a concentrate as well. T
I have ordered mine from Cell Marque. We use it at 1/50 dilution with a 30
minute incubation at RT on the Dako Autostainers. Works great.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
__
:30 AM
To: jtay...@meriter.com; ih...@googlegroups.com;
histonet@lists.utsouthwestern.edu
Subject: [IHCRG] Re: [Histonet] Helicobacter pylori IHC stain
We use Biocare's Rabbit Polyclonal (1:150) with the red
chromagenreally cleaned up all the background..the docs love it!
Ro
We are also trying Biocare's mouse monoclonal with red chromagen. Organisms
look like HP on steroids!
Beautiful...
Konni Black
- Original Message -
From:
To: ; ;
Sent: Thursday, November 12, 2009 7:30 AM
Subject: Re: [Histonet] Helicobacter pylori IHC stain
We use Bioc
We use Biocare's Rabbit Polyclonal (1:150) with the red chromagenreally
cleaned up all the background..the docs love it!
Roxanne
-Original Message-
From: Taylor, Jean
To: 'ih...@googlegroups.com' ;
'histonet@lists.utsouthwestern.edu'
Sent: Tue, Nov 10, 2009 4:41 pm
Subject:
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