The exact cutting protocol should always be developed with your pathologists.
That said:
8 slides, step sections, multiple sections (as many as the size of specimen 
will allow) per slide.
Slides 1, 3, 4, 6, 8 - H&E
Slides 2, 5, 7 - unstained/held for additional staining (SS/IHC)

William DeSalvo, B.S., HTL(ASCP)

 


> From: allison_sc...@hchd.tmc.edu
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 11 Jan 2012 16:27:34 +0000
> Subject: [Histonet] Hirschprung Protocol
> 
> Hello to all in histoland. Does anyone have the cutting protocol for a 
> hirschprung biopsy. Your help in this matter will be greatly appreciated.
> 
> 
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital
> 
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