either,
has anyone figured this out?
Esther
From: histonet-boun...@lists.utsouthwestern.edu
on behalf of John Kiernan
Sent: Tuesday, January 21, 2014 8:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Histogel
The document at
http://lists.utsouthwestern.edu/mailman/li
dusko trajkovic
> Sent: Monday, January 20, 2014 12:47 PM
> To: Esther C Peters; jennifer.arcand-john...@genzyme.com;
> histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Histogel
>
> Esther,
> I mainly process cells, which have been spun down into a small pellet. Also
> mou
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histogel
Esther,
I mainly process cells, which have been spun down into a small pellet. Also
mouse DRG's and other very small tissues. I would consider this delicate, so do
not be afraid to use a longer processing program. Histogel/
her C Peters
To: "jennifer.arcand-john...@genzyme.com"
; "histonet@lists.utsouthwestern.edu"
; dusko trajkovic
Sent: Monday, January 20, 2014 11:15 AM
Subject: RE: [Histonet] Histogel
Thank you, Dusko!
I have had the same problem with 1.5% agarose, and I tried starting
ehalf of dusko trajkovic
Sent: Monday, January 20, 2014 1:58 PM
To: jennifer.arcand-john...@genzyme.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histogel
Jennifer,
You might have seen one of my posts from 2-3 years ago. I had the exact same
problems you described. Could not get anyone t
Jennifer,
You might have seen one of my posts from 2-3 years ago. I had the exact same
problems you described. Could not get anyone to come up with a solution. I ran
various programs on our VIP and finally came up with a solution.
Fix your specimens as you normally would do. Drain of the fixativ
Amos,
I have had the same problem in the past, and posted my issues on the Histonet,
however no one was able to help me out. At one point I even exchanged Histogel
with a colleage severl hundred miles away, thinking that maybe I had a bad lot
of Histogel. She did not have a problem with my Histo
Hi,
I've encountered this problem before in my previous lab. To reduce the
Histogel from shrinking that badly, avoid putting the tissue at the very edge
of the Histogel, space them out nicely in the middle, providing sufficient
amount of extra Histogel between each tissue and surrounding them.
A little bit of plain agar, or a plain TSA slant tube (stolen from
micro), melted in the microwave for <10 seconds, works just as well as
histogel, imho.
Jay A.
Lundgren M.S., HTL (ASCP)
> histogrl backlash
thats a whole 'nother set of issues altogether ;)
happy friday!
anh2...@med.cornell.edu wrote:
Wow, what is the histogrl backlash? Will someone please summarize the issues
people are having?
Thanks!
Andrea
-Original Message-
From: "Hofecker, Jennifer L"
Date:
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