: 'Morken, Timothy'; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu;
'Jason McGough'
Subject: RE: [Histonet] Humidity levels and IHC staining
NY in winter has low humidity, I still have no problems with my Dako
stainer. Make sure the lid is closed. I had a tech who
...@lists.utsouthwestern.edu] On Behalf Of Morken,
Timothy
Sent: Friday, February 17, 2012 2:27 PM
To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough
Subject: RE: [Histonet] Humidity levels and IHC staining
Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying
out. South
, 2012 2:27 PM
To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough
Subject: RE: [Histonet] Humidity levels and IHC staining
Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying
out. South Dakota, in winter? 10 percent humidity and you get dryin
Jason,
We've experienced similar problems a few summers ago when the environmental
conditions inside the Autostainers (dako) reach high temperatures and low
humidity. We keep thermometers inside the stainers to monitor these levels.
To remedy this problem, before every IHC run, I add a few d
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 17, 2012 11:20 AM
To: histonet@lists.utsouthwestern.edu; Jason McGough
Subject: Re: [Histonet] Humidity levels and IHC staining
A good auto stainer (like DAKO) with adequate amounts
[mailto:rjbu...@yahoo.com]
Sent: Friday, February 17, 2012 12:20 PM
To: histonet@lists.utsouthwestern.edu; Jason McGough
Subject: Re: [Histonet] Humidity levels and IHC staining
A good auto stainer (like DAKO) with adequate amounts of dispensed
reagents during the correct periods of
A good auto stainer (like DAKO) with adequate amounts of dispensed reagents
during the correct periods of time should not experiment any drying out on the
slides. Adequate humidity is required to be controlled during manual IHC,
especially if done over a heated support. If because of any reason
