RE: [Histonet] IHC-start-up

2014-01-24 Thread Edwards, Richard
Your biggest problem is not your choice of machine but which antibodies will work/cross react on animal tissues, many if not most, do not. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin

Re: [Histonet] IHC-start-up

2014-01-23 Thread Will Chappell
Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. I would steer clear of any platforms that do not let

Re: [Histonet] IHC-start-up

2014-01-23 Thread Colleen Forster
I totally agree with William. I do animal IHC and I am, using the Biocare Nemesis, which be the same as the Dako platform. Completely open so you can do what you need to at a cost you can afford Colleen Forster HT(ASCP)QIHC u of MN On 1/23/2014 11:44 AM, Will Chappell wrote: Doing ihc on

Re: [Histonet] IHC-start-up

2014-01-23 Thread Mike Thompson
Diagnostic Biosystems has the same platform with tech support that is outstanding! Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office Fax: 412-727-6080 IHC Made Affordable www.dbiosys.com Colleen Forster cfors...@umn.edu wrote: I

RE: [Histonet] IHC-start-up

2014-01-23 Thread James Watson
Subject: Re: [Histonet] IHC-start-up Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. I would steer clear

Re: [Histonet] IHC-start-up

2014-01-23 Thread Jan Shivers
9:45 AM To: Erin Sarricks Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC-start-up Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book

RE: [Histonet] IHC-start-up

2014-01-23 Thread Bitting, Angela K.
1:10 PM To: Colleen Forster Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC-start-up Diagnostic Biosystems has the same platform with tech support that is outstanding! Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office

RE: [Histonet] IHC-start-up

2014-01-23 Thread pruegg
Subject: Re: [Histonet] IHC-start-up Diagnostic Biosystems has the same platform with tech support that is outstanding! Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office Fax: 412-727-6080 IHC Made Affordable www.dbiosys.com Colleen