Use a formic acid based decal solution and MAKE SURE the specimen does not
fix (formalin fixation) longer than 32hrs (false negatives result). Use 70%
ETOH to manage the fixation time (ie fix for 24 hours, decal for 4 hrs,
place in 70% ETOH until it gets put on the processor. Hold in 70% ETOH for
w
Hi Kendra-
Decal Stat from Statlab is HCl based, therefore is not appropriate for
subsequent IHC, ISH, or other molecular studies. There is lots of
peer-reviewed published data, if you need to supply rationale to your
pathologists. HCl is known to significantly reduce molecular assay sensitiv
That may be what you have to do, as the first day of ISH needs to be RNase
free!
When I do them, it is 10 slides at a time and we have dishes and a lab
bench set aside for RNase free procedures only. Obviously this is not
feasible in all workplaces!
I would just think about it logically. Use glov
Histotechnician
<= /em>
XBiotech USA I= nc.
8201 East Riverside Dr. Bldg 4 Suite 100
Aust= in, Texas 78744
(512)386-5107
Original Message --------
Subject: RE: [Histonet] ISH on histogel cell pellets
From: "Liz Chlipala" <[1]...@premie= rlab.c
Kim the histogel should not interfere with the IHC staining, I'm not sure about
the ISH
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway
Try Novocastra and Bond Reagent products 2009. 800-248-0123 ext. 7880.
I hope this helps!
Josie Britton HT
Cheshire Medical Center
Keene, NH 03431
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behal
We use Leica - works perfectly!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Tuesday, August 11, 2009 14:36
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] ISH
Can anyo
Ventana has put their Kappa/Lambda back on the market in ASR format.
Jodie Robertson, HT(ASCP)QIHC
Histology Day Supervisor
Pathology Sciences Medical Group
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of W
There's a wonderful company called Oligo's, Etc. (Wilsonville, OR) that
synthesizes very affordable oligoprobes. But you have to supply the
sequence information. Susan
- Original Message -
From: "Sharon Campbell"
To:
Sent: Monday, January 26, 2009 9:20 AM
Subject: [Histonet] ISH pr
Yes, we cannot get our Her2 ISH from Ventana.
Laurie
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Patti
Loykasek
Sent: Tuesday, September 23, 2008 1:01 PM
To: histonet
Subject: [Histonet] ISH supplies from vendors
I was wondering if anyone else has ex
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