Images of IgG4-positive cells in tonsil FFPWS on Histonet Images website
Carl Hobbs FIBMS
Histology and Imaging Manager
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Kings College London
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We are using the MRQ-44 from Cell Marque.
From: Cartun, Richard via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Monday, January 11, 2016 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IgG4
I am curious to hear which clone people
Hi Richard- I have been using the CellMarque IgG4 (MRQ-44 clone), mouse
monoclonal on the Leica Bond with success.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
-Original Message-
From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
We use Cell Marques Mouse monoclonal (clone MRQ-44) Cat# 367M-16 at a 1:200
dilution on the Ultra. We are using the Optiview detection kits.
CC#1-24 min
Option 5 - 12 min
Antibody - 24 min
OV Linker 8 min
Multimer 8 min
H2O2 +DAB 8 min
OV copper 4 min
Hematozylin II 8min
bluing 4 min
We onl
Cell Marque now has an IgG4 IVD clone. It is one of the only ones I have
found.
On Tue, Jan 4, 2011 at 11:13 AM, Shields, Kim wrote:
> Looking for IgG4 for IHC-paraffin application. We were using it from
> Invitrogen, but apparently, it is no longer available. Any help is much
> appreciated.
I have used invitrogen - mouse anti-human IgG4 Clone HP6025 (successfully)
AR in 1mm EDTA Dilution 1:400
Control - tonsil (or Sjogren's Syndrome)
>>> Joao Pessoa 2010/05/07 11:50 PM >>>
I need to find a source for an IgG4 antibody for use with IHC studies on
human FFPE tissue. Any ideas? My
Dear Karen,
We are using Invitrogen's monoclonal Anti-human IgG4 (Cat No. 05-3800) with
a working dilution around 1/700.
Although we are using BondMax stainer with 30 min, Epitope Retrieval
solution 2 (alkaline) pretreatment from Leica, Ventana would give a good
staining as well.
Young
Sun You
