Classification: UNCLASSIFIED Caveats: NONE We use "Distilled White Vinegar". I buy it at the grocery store by the gallon. It is the acetic acid in bouin's solution that fixes the ink to the tissue, not the picric acid. I have been using white vinegar for more years than I care to remember. Hope this helps.
Diane Gladney Supervisor, Histology Dept. Moncrief Army Community Hospital Ft. Jackson, SC -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is inked into Bouins solution, and that they didn't have any issues with inks where he came from. I have heard of this technique before, But I was just able to get rid of all of the bounins solutions in the lab and I am hesitant to bring it back, looking for Ideas and thought from all, also if you use the Bouins solution to "fix" your inks- I would love to learn your thoughts about the process. Thanks-a-million September Amspacher HT(ASCP) Technical Specialist- Histology Department Laboratory Chemical Hygiene Officer Bassett Healthcare Cooperstown, New York _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet