Re: [Histonet] Manual embedding

2011-08-09 Thread Collette, Nicole M.
Hi, All, I just saw this question and the responses, thought I would add my own solution to the mix. I do manual embedding, I use a hyb oven for my infiltrating/embedding station (like for hybridizing Southern or Northern blots- who does that anymore?). I have taken out the rotating wheel for

RE: [Histonet] Manual embedding

2011-08-04 Thread Hugh Luk
Scott, Pacific Southwest Lab Equipment in California has been good to me. Very fair prices compared to new. However, I also must state that two of the four items purchased Used from them no longer work (embedder, cryostat) BECAUSE of the new freon regulations. Be aware that in

Re: [Histonet] Manual embedding

2011-08-03 Thread Andrea Marion
Hi Scott, I work in a research lab that does manual embedding too. I looked into purchasing a paraffin pitcher, but it was too expensive for us. Our makeshift embedding center is comprised of: 1) 3 thick-walled plastic 500 ml beakers covered in a double layer of foil (you could use glass, but it

Re: [Histonet] Manual embedding

2011-08-03 Thread Stella Mireles
Andrea, I have send you an idea that might work for you. Check your email. Hope this will help. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Manual embedding

2011-08-03 Thread Rene J Buesa
...@gmail.com wrote: From: Stella Mireles estellamire...@gmail.com Subject: Re: [Histonet] Manual embedding To: Andrea Marion amar...@uic.edu Cc: histonet@lists.utsouthwestern.edu Date: Wednesday, August 3, 2011, 4:29 PM Andrea, I have send you an idea that might work for you. Check your email. Hope

Re: [Histonet] Manual embedding

2011-08-03 Thread Stella Mireles
A couple of ideas: Suggest looking for a used paraffin bath from an outside vendor. #2 Idea: If anyone knows of a lab that is closing up, they might be able to contact Andrea and pass that info. I know when a lab is closing up, storage of equipment becomes burdensome.