ate: Wed, 31 Aug 2011 09:20:09 -0400
> From: talulahg...@gmail.com
> To: carmen.m.gar...@uv.es; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Mice embryo cryocuts
> CC:
>
> You need to leave the embryos in sucrose waaay longer than 5 hours. I would
> leave them at
You need to leave the embryos in sucrose waaay longer than 5 hours. I would
leave them at least overnight, if not more than that. You can probably take
them out of the blocks and resink them in sucrose, if you're desparate.
But next time, always sink (ie wash in sucrose) for at least overnight.
