Possibly you have allowed the slides to air dry too long before staining. After cutting and allowing to dry for 10 minutes, store them at -70oC prior to staining might solve the problem
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, 4 February 2009 6:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Muscle Gomori Trichrome Hi Histonians, I have experienced some difficulties in doing snapped freeze muscle biopsies that need to be stained with the Modified Gomori's Trichrome by Engel, W.K., and Cunningham 1963. When I stained the frozen tissue that has not been post or pre fixed, I am getting red areas within this bundles that also have green in it. I know it should be solid green with reddish nuclei and red intermyofibrillar muscle. I truly believe this is an artifact. I checked the histonet archives and there has been much talk about the stain but not how to correct this artifact Any help will be appreciated. John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet