...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey
[sa...@hotmail.ca]
Sent: Friday, August 24, 2012 9:50 AM
To: billodonn...@catholichealth.net; rjbu...@yahoo.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Osmium tetroxide staining for lipids
Thank
Sheila, You can't normally use osmium lipid fixation for paraffin sections
because the lipid you want to see will usually have been dissolved out by the
paraffin processing. You need to either post-fix in osmium (after formalin)
before paraffin processing or use frozen sections. As mentioned, on
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, August 24, 2012 10:32 AM
To: Sheila Adey; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Osmium tetroxide staining for lipids
Osmium tetroxide is one of the most dangerous substances you can use in the
Two other arguments against osmium, it is very expensive and is must be
disposed of as hazardous waste.
Geoff
On 8/24/2012 10:50 AM, Sheila Adey wrote:
Thank you so much for this response. :)
Subject: RE: [Histonet] Osmium tetroxide staining for lipids
Date: Fri, 24 Aug 2012 08:42:34
Thank you so much for this response. :)
> Subject: RE: [Histonet] Osmium tetroxide staining for lipids
> Date: Fri, 24 Aug 2012 08:42:34 -0600
> From: billodonn...@catholichealth.net
> To: rjbu...@yahoo.com; sa...@hotmail.ca; histonet@lists.utsouthwestern.edu
>
> I agree
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R
Sent: Friday, August 24, 2012 9:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Osmium tetroxide staining for lipids
...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, August 24, 2012 9:32 AM
To: Sheila Adey; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Osmium tetroxide staining for lipids
Osmium tetroxide is one of the most dangerous substances you can use in the
laboratory. Your pathologist
Osmium tetroxide is one of the most dangerous substances you can use in the
laboratory. Your pathologist probably read some article or found an old photo
of fat "stained" with osmium tetroxide and now wants you to do the same thing.
The problem is that the fat is allowed to react to the fumes of
Sheila Hi
I am assuming that you are using frozen sections?
The easiest way is to put a small amount of aqueous osmium tetroxide in the
bottom of a dish that has a tight fitting lid.
Place the slide with section into the dish but not touching the fluid.
Place tightly fitting lid and leave - not su
