t 2008 9:09 am
Subject: RE: [Histonet] PAS with Glycogen Digestion - Solution Not Working
Diastase powder will dissolve in any water, but requires some calcium ions
o function. Thus it will not perform in distilled water, but will perform
ell using plain tap water. Also, the diastase powder from
[mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa
Sent: Friday, October 24, 2008 8:38 AM
To: histonet@lists.utsouthwestern.edu; McKnight, Tanisha
Subject: Re: [Histonet] PAS with Glycogen Digestion - Solution Not Working
Tanisha:
Being an enzyme, you cannot use diastase in distilled water. You
I had far more consistent results with amylase type VI (sigma) than diastase,
recommended by someone from histonet when I had problems with diastase
Ren Fail
-- Original message from "McKnight, Tanisha" <[EMAIL PROTECTED]>:
--
> Hi All:
>
> I am in the process of
Tanisha:
Being an enzyme, you cannot use diastase in distilled water. You have to
prepare a diastase buffer at pH7 to dissolve the diastase and do the digestion
at 37ºC during 30 minutes. That will work.
Please, do not try to use saliva!
René J.
--- On Fri, 10/24/08, McKnight, Tanisha <[EMAIL PR
