I use the Biocare polymers all the time because they are cheaper than
DAKO. Some of them are biotin free, so as long as your primary has no
biotin and you aren't staining anything with endogenous biotin you can
omit that block. Other than that enzyme and protein blocking I still
do. Some of them
Amita,
I know there are others out there with much more immuno experience than myself,
but I have been working up procedures on animal tissue for a few years now, so
I can give you my opinion. I have always had good results with Biocare
products. I would try their polymer kit. Vector impress
Anita
Since you are working on rat tissue, you have to be careful on what detection
systems you use. You will need two different detection systems for your work.
One for the mouse monoclonal antibodies and one for the rabbit polyclonal. If
you want to use a polymer based detection system th
I personally love polymers!! The DAKO envision is actually using
polymers. You don't have to buy the kit. Biocare has a ton of awesome
polymers for sale that I use in tangent with DAKO. If you want more
specifics on my protocols let me know.
Good Luck
Sarah Goebel, BA, HT(ASCP)
Histotechnologi
