, mostly with snap freeze
or meoh/acetone fixed tissues without cryoprotectant.
Hope this helps.
-Mehlika
> Date: Fri, 7 Oct 2011 14:36:24 +1100
> From: julie.bil...@gmail.com
> To: pbo...@histoprep.com
> Subject: Re: [Histonet] Poor quality frozen sections, feline skin.
&g
Hi Peter,
We use 20-25% sucrose on our fresh specimens for immunofluorescence (IF). We
only deal with human skin samples so I will not comment on feline skin. I
sit the specimen in three changes of sucrose for 10 minutes each. Before
embedding in the cryostat, I gently blot it on a tissue. My unde
