We use Surfactant from Biocare at 1/100 dilution 30 minutes incubation, but we use a lower pH retrieval 6.0 for 20 minutes at 99C. I haven't had any trouble with the Cyclin D from Dako, we use the Ready to Use version though. Hope that helps.
Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Karla Arrington [freckles9...@yahoo.com] Sent: Monday, May 24, 2010 10:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems w/IHC's Hello Histo's: We are having troubles with getting our Cyclin D1 to stain for longer than one month. I seems to whimp out after a month, so we make up fresh. Any ideas? We are using a Dako clone. Also we are currently working up Surfactant A with little luck. Our lung control does not stain at all while our patient panel is weak. We are doing 1:00 dilution for 30 mts and 30 mts using high pH (9.9) for retrieval. Any help would be greatly appreciated!! Karla Arrington HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
