I have not used "Chicago Blue" (Inoticed the product is discontinued by Sigma Aldrich) for quenching, but have often used "Evans Blue" (synonym: Direct Blue 53; Empirical Formula: C34H24N6Na4O14S4 ;Molecular Weight: 960.81;CAS Number: 314-13-6) without any issue. Sigma Aldrich sells the Evans Blue is solution (>75% dye content). You might want to give the Evans Blue a try (product # E2129).
William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 18 Jan 2012 16:11:51 +1100 > From: daniela.bode...@mcri.edu.au > To: Histonet@lists.utsouthwestern.edu; > histonet-requ...@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Quenching auto fluorescence in human tissue > > Hi all, > > > > I have been experiencing trouble with my secondary antibody when doing > my immuno with human colon tissue. > > I use to use FITC 488 as my secondary antibody and changed over to > Alexa488 due to the FITC's manufacturing stop. > > The auto fluorescence of the human tissue is quenched with Chicago Blue > solution and the slides are mounted with Prolong Gold antifade reagent > to extend the life of the immuno slides. > > The trouble is, after a few days the fluorofors seem to 'detach' and > start floating around, not allowing reliable validation of the results. > This also happen if I use Mowiol as a mounting medium. > > Is the mounting medium reacting with Chicago Blue? Is there any > alternative to quench die auto fluorescence of the tissue? > > > > Thanks for your ideas. > > > > Daniela Bodemer > > Research Assistant > > Surgical Research, Infection and Immunity > > > > Murdoch Childrens Research Institute > > The Royal Children's Hospital > > Flemington Road Parkville Victoria 3052 Australia > > T 03 9936 6676 T (03 9936 6794) > > E daniela.bode...@mcri.edu.au <mailto:firstname.surn...@mcri.edu.au> > > www.mcri.edu.au <http://www.mcri.edu.au/> > > > > This e-mail and any attachments to it (the "Communication") are, unless > otherwise stated, confidential, may contain copyright material and is > for the use only of the intended recipient. If you receive the > Communication in error, please notify the sender immediately by return > e-mail, delete the Communication and the return e-mail, and do not read, > copy, retransmit or otherwise deal with it. Any views expressed in the > Communication are those of the individual sender only, unless expressly > stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 > 006 566 972 or any of its related entities. MCRI does not accept > liability in connection with the integrity of or errors in the > Communication, computer virus, data corruption, interference or delay > arising from or in respect of the Communication. > > P Please consider the environment before printing this email > > > > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
