Try soaking the face of the block after facing on a wet ice-cube tray! Josie Britton HT/QIHC(ASCP) Cheshire Medical Center Keene, NH 03431
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Thursday, February 14, 2013 6:35 PM To: 'Metzger, Kenneth'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: "Vacuole" appearance in lymph Nodes Ken, Are the Microtomists being over enthusiastic with the block "trimming" or "facing"? This is more prone to happen if the block is cold (ie harder) during trimming. I would recommend after trimming to full-face that a few slower turns of the microtome wheel at 4-5 microns will clear the holes (if this is indeed the cause of the holes) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, 15 February 2013 3:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "Vacuole" appearance in lymph Nodes Hi All, I have sent this question to respected colleagues individually so pardon my redundancy if you have this email already. Lately we have had random nodes show up with areas that appear to have multiple vacuoles through-out the specimen. They have different patterns section to section so I know it is not in the tissue. The staining looks fine and the tissue cuts well so I don't think it is a processing issue. Any ideas on the problem would be welcome. Thanks Ken Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metz...@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ********* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ************************************************************************ ********* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet