The most important part= about working up a new antibody is to have
known positive tissue to use as= a control, if you do not have that
everything you do is a shot in the dark= , you will not know if your
protocol is not working or the control you are = using is not
Yes, I have, redoing the AR does fix that though.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
This email is confidential and intended
hold in PBS as long as several days without any problem, under 4 C.
2009-10-22
TF
发件人： Perry, Margaret
发送时间： 2009-10-22 04:14:31
收件人： histonet@lists.utsouthwestern.edu
抄送：
主题： [Histonet] RE: Antigen retrieval question
If we need to hold retrieved slides for awhile we hold them in
Thanks to all who replied to my question.
It is not our usual procedure to leave slides overnight in buffer after
AR and not one that we will ever repeat.
Brett
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 f
