RE: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

2011-04-14 Thread Tony Henwood
To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should

Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

2011-04-13 Thread Jay Lundgren
As long as possible. I remember reading somewhere about TB in lung tissue that was still viable after *years* of 10% NBF. Make sure the lungs are thoroughly perfused, or they will still be unfixed in the middle. There is a technique to inflate lungs at autopsy using a large syringe full of

Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

2011-04-13 Thread Rene J Buesa
5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions. René J. --- On Wed, 4/13/11, Thurby, Christina christina.thu...@bms.com wrote: From: Thurby, Christina christina.thu...@bms.com Subject: [Histonet] RE: How long should autopsy/necropsy tissue

RE: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

2011-04-13 Thread Kumar, Devender
: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 13, 2011 3:57 PM To: histonet@lists.utsouthwestern.edu; ChristinaThurby Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation