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> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
> pru...@ihctech.net
> Sent: Wednesday, October 02, 2013 9:36 AM
> To: Barbara Tibbs
2013 9:36 AM
To: Barbara Tibbs; Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] RE: MOHS IPs
I agree with Barbara try fixing in cold acetone/ethanol mix even =
just for a couple of minutes then go directly to buffer do not dry
after=x, I woul
I agree with Barbara try fixing in cold acetone/ethanol mix even = if
just for a couple of minutes then go directly to buffer do not dry
after= fix, I would dilute the protease 1:4 with buffer (PBS or TBS
what ever u u= se) and do it for a short time. Enzyme digestion on
