I have the bond and I use the BIO SB concentrate, RaBMab, EP190 at 1:50 and it stains great on tonsil and kidney tubules.
Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lseb...@uwhealth.org > To: cda...@che-east.org; histonet@lists.utsouthwestern.edu > Date: Thu, 13 Mar 2014 16:19:11 +0000 > CC: > Subject: [Histonet] RE: MUM-1 > > This is our protocol for the Ultra; maybe it will help. > > 64" CC1, 32" incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4". This is > with UltraView DAB detection. We use tonsil as well however we validated > with HD, LN, GI, tonsil, etc. > > Good luck! > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > Sent: Thursday, March 13, 2014 8:54 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] MUM-1 > > Good morning Histonet Folks, > > I am hoping one of you will help me. I am in the process of > optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the > Benchmark XTstainer and I am not thrilled with the results I am getting. I > have tried the "usual adjustments" and the results are less than optimal in > my opinion. I am using a normal tonsil control right now but if you have > another suggestion please do not hesitate to recommend. I am praying somebody > might have done this before and would be willing to share their staining > protocol or tips with this. > > Cassandra Davis > cda...@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health > East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > are not the intended recipient, please delete this message, and reply to the > sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
