Kathleen
I have a final volume of 10 mls not 1 ml in the equation I wrote.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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When I solve your equation V1 X 1560 ug/ml = 10ml X 0.8 ug/ml I get .005 ml.
Therefore the dilution would be closer to 1:2000. I would usually divide the
1560 ug/ml by 0.8 ug/ml and get 1950 therefore 1:1950 would be more accurate .
Tom T
-Original Message-
From:
; Sheryl Stephenson; histonet@lists.utsouthwestern.edu;
Elizabeth Chlipala
Subject: RE: [Histonet] RE: lab math: ihc dilution
When I solve your equation V1 X 1560 ug/ml = 10ml X 0.8 ug/ml I get .005 ml.
Therefore the dilution would be closer to 1:2000. I would usually divide the
1560 ug/ml by 0.8 ug
@lists.utsouthwestern.edu)
Subject: RE: [Histonet] RE: lab math: ihc dilution
You are right I got it wrong, see no matter how many time you run the math, you
still come up with problems
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592
Boulder, CO 80308
(303) 682-3949 office