Re: [Histonet] RE: Paraffin Temperature Checks

2015-01-08 Thread b427297
I like to use a chart recorder on paraffin dispensers in case the thing goes whacko over a weekend and boils the paraffin, then goes back to normal before anyone notices. Sent from my iPhone > On Jan 8, 2015, at 6:10 PM, Elizabeth Chlipala wrote: > > All of our logs are use logs so we record

Re: [Histonet] RE: paraffin

2014-08-14 Thread Jay Lundgren
New (or unparaffined, to coin a term) shoes are the major threat here, imho. When one has paraffin on the bottom of one's shoes and steps on more paraffin, it is kind of sticky. But if you wear new shoes, or shoes that have never been in the histo lab, look out! Hence Gayle's experience with the

RE: [Histonet] RE: paraffin

2014-08-14 Thread gayle callis
I want these in doorways of my house. We used tacky mats at entrance of designated room for prion tissue work i.e. paraffin microtomy and cryomicrotomy, etc. Fabulous way to keep the floors clean. We also had industrial rugs i.e. the kind seen at entrances to large buildings in lab where r

Re: [Histonet] RE: paraffin embedding mold for rat eye

2012-01-19 Thread Grantham, Andrea L - (algranth)
You could contact Simport. A few years ago I got some deeper disposable molds from them and they work great with tissues that are too high for the regular molds (like the Tissue-tek molds). The only problem is that they only came in the large width and you waste a lot of paraffin. Another though

Re: [Histonet] RE: paraffin sections: white with airbubbles

2011-02-02 Thread amitapandey
another piece of fixed tissue , process it with standard paraffin processing protocol. Dr. Amita Dubey PCSED,TRC From: John Kiernan To: "Thomas, Nancy" Cc: "histonet@lists.utsouthwestern.edu" , 'An Eerdekens' Date: 02/02/11 12:28 PM Subject: Re: [Histonet] RE:

Re: [Histonet] RE: paraffin sections: white with airbubbles

2011-02-01 Thread John Kiernan
You can't go from 70% alcohol into paraffin without passing through 100% alcohol and then a clearing agent (liquid miscible with 100% alcohol and with melted wax). Xylene is a commonly used clearing agent. Your times in 50% and 70% alcohol are much longer than necessary. Even for a whale's hypot

RE: [Histonet] Re: Paraffin Blocks

2009-02-06 Thread Bernice Frederick
Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, February 05, 2009 5:13 PM To: Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Paraffin Blocks I've been prea

RE: [Histonet] Re: Paraffin Blocks

2009-02-06 Thread Weems, Joyce
e of my 2 cents!!! j From: Bonner, Janet [mailto:janet.bon...@flhosp.org] Sent: Friday, February 06, 2009 8:50 AM To: Weems, Joyce; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Paraffin Blocks We are a very large facility and kept the majority of our bl

RE: [Histonet] Re: Paraffin Blocks

2009-02-06 Thread Bonner, Janet
: [Histonet] Re: Paraffin Blocks I've been preaching this sermon for several years!!! We must do something!!! j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, Februa

RE: [Histonet] Re: Paraffin Blocks

2009-02-05 Thread Weems, Joyce
I've been preaching this sermon for several years!!! We must do something!!! j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, February 05, 2009 5:28 PM To: histonet@lists.

Re: [Histonet] Re: Paraffin Blocks

2009-02-04 Thread Rene J Buesa
In Florida we need to keep blocks only during 9 years. Every year we disposed those kept for 9 years and started a new series (using the same cabinets). Other states may have different regulations. René J. --- On Wed, 2/4/09, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: [Histone

RE: [Histonet] Re: Paraffin Blocks

2009-02-04 Thread Pamela Marcum
We contact the person who requested and submitted the specimens to find out if they want the blocks or tissue in some cases. If they no longer want them, have moved on to another facility or graduated and gone so are the blocks. The only other person we ask is the overall PI of the laboratory and